2024 Hcs lipidtox

Hcs lipidtox

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August undefined, 2024

WebHcs Lipidtox Deep Red Neutral Lipid Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … WebHCS LipidTOX™ Deep Red, 1000 X. Names; Short Name: HCS LipidTOX™ Deep Red. Synonym(s) HCS LipidTOX™ Deep Red. InChI Key (linked to PubChem) N/A. …

Methods to Assess Lipid Accumulation in Cancer Cells

WebA collection of spectra representing the microscope Nikon Ti2 on FPbase. WebExcess lipid accumulates in vesicles that displace the cytoplasm; large accumulations of lipids can disrupt cell constituents, and in severe cases, the cell may even burst. Steatosis is a common consequence of hepatocellular insult, which can be drug-induced or can be an outcome in pathophysiogical disease progression. dexcs openfoam インストール

Different Sensitivity of Macrophages to Phospholipidosis Induction …

WebHCS LipidTOX Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to characterize phospolipidosis and steatosis, toxic side effects on lipid metabolism that can be triggered by drugs or other compounds. The kit supplies LipidTOX Red phospholipid stain and ... WebDec 17, 2016 · After 48 culture140 Glocket al. Vitro35 (2016) 139–148 mediumwas replaced freshmedium, including HCS LipidTOX Green phospholipidosis detection reagent (LipidTOX) (Invitrogen, Carlsbad, US) (1:1000), respectiveconcentration bina-ry combinations Samplescontaining LipidTOX respectivesolvent(s) (vehicle controls) were … WebJan 1, 2014 · HCS LipidTOX Red staining. This section describes cell staining with HCS LipidTOX Red (Invitrogen). Alternative colors of the same reagent and similar reagents from other sources are available and should work just as well. HCS LipidTOX Red can also be applied to live cell imaging. 1. Fix cells (see Section 3.5) and remove the PBS. 2. lasse hyytiäinen

Metformin induces lipogenic differentiation in myofibroblasts to ...

Invitrogen™ HCS LipidTOX™ Green Neutral Lipid Stain, for cellular ...

WebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and … WebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and … lasse jokinenWebInvitrogen™ HCS LipidTOX™ Green Neutral Lipid Stain, for cellular imaging. High affinity for neutral lipid droplets. Brand: Invitrogen™ H34475. Code : 50. Additional Details : … lasse jermiin

"WebJan 13, 2024 · Then, cells were stained with HCS LipidTOX Red neutral lipid stain (H34476, Thermo Fisher Scientific) for 30 min. Nuclei were stained with DAPI for 5 min. All slides were mounted with VECTASHIELD ... " - Hcs lipidtox

Hcs lipidtox

Acetylation Stabilizes ATP-Citrate Lyase to Promote Lipid …

WebFeb 7, 2024 · Images confirm that the mTOR inhibitor AZD8055 accentuates puncta formation revealed by the HCS LipidTox probe (Fig. 3c), suggesting that TMEM41B depletion and mTOR inhibition act synergistically ... Web11668019，lipofectamine 2000 reagent 脂质体2000转染试剂

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WebOct 30, 2024 · HCS LipidTOX™ Phospholipidosis and Steatosis detection kits; The kit has following components (a) Red phospholipidosis detection reagent (1000× stock). (b) … WebDec 7, 2024 · Lastly, we used high-content screening (HCS) to determine the levels of reactive oxygen species (ROS), lipid, and mitochondrial membrane potential (MMP). Results: The ethanol extraction process aggravated the hepatotoxicity of …

WebCitations & References The HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid … WebHCS LipidTOX™ 中性脂质染料可用于成像法高内涵筛选（HCS）检测，可表征化合物对哺乳动物细胞系中脂质代谢的潜在毒副作用。 LipidTOX™ 系列检测试剂盒包括用于固定细胞的中性脂质染料和用于活细胞的磷脂染料，两者可在多重分析中组合使用。选择 HCS LipidTOX™ 染料骨髓来源的 hMSC 脂质囊泡，用 LipidTOX™ Green 中性脂质染料染 …

WebOct 30, 2024 · However, the combination of HCS LipidTOX™ steatosis reagents provides a labeling strategy to perform high content imaging multiplexed assays to characterize phospolipidosis and steatosis, and to measure the effects on lipid metabolism that can be triggered by drugs or other compounds. WebHCS LipidTOX Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to …

WebFeb 7, 2024 · Images confirm that the mTOR inhibitor AZD8055 accentuates puncta formation revealed by the HCS LipidTox probe (Fig. 3c), suggesting that TMEM41B depletion and mTOR inhibition act synergistically to amplify lipid accumulation in the observed puncta. We note that the most significant increases in DUNN index were …

WebHCS LipidTOX™ Deep Red, 1000 X. Names; Short Name: HCS LipidTOX™ Deep Red. Synonym(s) HCS LipidTOX™ Deep Red. InChI Key (linked to PubChem) N/A. Description; Category: Stain; MW: N/A. Stock Concentration: 1000 X. Preparation; Solvent for Stock Solution: DMSO. Storage; Temperature-20°C; Precaution(s) Protected from light; lasse laaksonenWebThermo Fisher hcs lipidtox green neutral lipid stain solution Hcs Lipidtox Green Neutral Lipid Stain Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more lasse kilpinenWebSep 26, 2024 · For time-lapse imaging, live cells were treated with different agents and HCS LipidTOX red neutral lipid dye (Invitrogen, 1:200) was immediately added. ... LipidTOX staining confirmed the increase in lipid-droplet accumulation in these cells (Fig. 6E, F). Thus, BMP2 is a positive regulator of lipogenic differentiation in human IPF lung ... lasse lehtinen murhattu ministeriWebHCS LipidTOX Red solution from the manufacturer µl/10 ml PBS). Cells in MatTek dishes were stained after fixation by addition of 2 ml solution of dye in PBS. To reduce the effects of temperature variation on stain-ing efficiency, we incubated the fixed cells in staining solution for 30 min at 37C. They were then immedi- lasse lukkarinenWebMar 28, 2015 · HCS LipidTOX™ Red Neutral Lipid stain has an extremely high affinity for neutral lipid droplets and is best imaged with filter sets appropriate for Alexa Fluor® 594 dye or Texas Red® dye (ex577-em609 nm). The XY coordinates were annotated in order to recognize the same microscopic field. Treble staining allowed us to evaluate possible ... lasse kristiansenWebJun 17, 2024 · Lipid droplets were separately analyzed by subtracting the HCS LipidTOX deep red stain from the HCS CellMask green stain to generate dark circular reliefs. These reliefs were automatically detected … dewks メリットWebAug 20, 2024 · To better characterize a potential role of TMEM41B in regulating LDs, we analyzed H4 cells basally or upon treatment with BSA-conjugated oleic acid by probing with HCS LipidTox Green Neutral Lipid Stain, a probe with high affinity for neutral LDs. dewx eterna ポートタイプm