WebApr 22, 2024 · Ala scan analogues were analyzed for their secondary structure content using circular dichroism (CD) spectroscopy. Their binding affinities were assessed qualitatively against BSA-conjugated monosaccharides (L-fucose, D-galactose, and D-GalNAc) and mucin (MUC1) glycopeptides carrying the Tn antigen using a fluorescence … Webalanine_scanning.py This script performs the alanine scanning mutations for residues in the binding site and is used by ABS-Scan (http://proline.biochem.iisc.ernet.in/abscan/). This script uses the libraries from Pymol, Modeller and Autodock tools to perform the analysis. These tools have been appropriately cited in the publication -
Alanine - an overview ScienceDirect Topics
WebJun 1, 2001 · Combinatorial libraries of alanine-substituted proteins can be used to rapidly identify residues important for protein function, stability and shape. Each alanine … http://www.mimotopes.com/peptideLibraryScreening.asp?id=94 cut crystal wine glasses/vintage
TCR Fingerprinting and Off-Target Peptide Identification
WebJun 3, 2016 · The Morphosys HuCAL GOLD ® library is a synthetic, fully human antibody library containing 1.2x10 10 different functional human antibody genes. This extremely large library of antibody molecules permits the recognition of a large number of foreign molecules. Alanine Scanning was used to determine simultaneously the functional contributions of 19 side chains buried at the interface between human growth hormone and the extracellular domain of its receptor. Each amino acid in the side chains was substituted by alanine. Then shotgun scanning method which combines the concepts of alanine scanning mutagenesis and binomial mutagenesis with phage display technology was used. WebFig 1. Alanine scanning library. Alanine scanning mutagenesis technology is a useful and powerful approach to construct the peptide library (alanine scanning library) for identifying the B cell epitopes. Because of its inert methyl functional group, Alanine is often used on our platform to characterize the contribution of a specific amino-acid. cut crystal wine glasses nz